NPQ: qE, qT, qI

Technical Support and Questions

NPQ include: qE qT qI. What do you think, how to assess the contribution of each parameter. Is that possible?
And how calculate value qP - photochemicalquenching? Thanks!

Thumb sebastian kuhlgert square


May 2016

In order to assess those parameters, you need to dark adapt your leaf, take measurements in the light, followed by dark recovery period. During each period you have to probe the fluorescence and give saturating pulses of actinic light (basically what you do with a simple ΦII measurement protocol, just multiple times). Those measurements take between 30-40 min. The publication below explains how the values are derived and what a measurement looks like (Figure 2).

Baker, N. R. (2008). Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu. Rev. Plant Biol. 59, 89–113. doi:10.1146/annurev.arplant.59.032607.092759.