Photosynthetic Rate in Legumes


Compare photosynthetic rates of nodulated v. N-fertilized legumes. Using Soybean, Medicago truncatula, Medicago polymorpha, and Trifolium fucatum.


Chlorophyll Fluorescence Phi2

Using an orange measuring light (pin 15) and red saturating and actinic light (pin 20), this performs the standard PAM fluorescence measurement but includes a background constant actinic light which is equivalent to the ambient PAR value.

Chlorophyll content (SPAD) I

REQUIREMENTS: Firmware v0.38 or lower (used for older devices which do not have a SPAD calibration, generally device ID 87 and lower).Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness.


  1. Plant Species (Multiple Choice)
  2. Leaf age (Multiple Choice)
  3. Nitrogen source (Multiple Choice)


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