Flood tolerance screening in southern US soybean


Large scale rain events are expected to become more common with the unpredictable effects of climate change. Flood tolerance is an important trait to look for in developing crop varieties that we depend on. In North Carolina especially, flooding of crop fields can cause billions of dollars in damage. A large scale field screening of soybean for flood tolerance is needed to study our unique growing conditions.

We are using the MultispeQ device to screen southern adapted soybean varieties and breeding lines for genetic flood tolerance in Plymouth, NC. A controlled flood treatment is imposed on plots by diverting fresh water into earthen berms surrounding the plots. MultispeQ measurements will be taken on treatment plots and control plots throughout the day to study chlorophyll and photosynthetic parameters.

Instructions: 1. Answer the questions on your phone. 2. Select 1 leaf from 3 random plants in the plot. 3. Select leaves from the newest completely expanded trifoliate (1 or 2 nodes down from top node). 4. Tap 'measure', then open and close the clamp on the side of the leaf to take a measurement!


Leaf Photosynthesis MultispeQ V1.0


Measures many photosynthesis-related parameters in <15 seconds, including:

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF
  • Relative Chlorophyll: SPAD
  • Proton Motive Force: ECSt, vH+, gH+
  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.
  • Leaf Thickness (in mm), angle, and cardinal direct...


  1. Which test are you measuring? (Multiple Choice)
  2. Plot # (Short Answer)
  3. Leaf # (Multiple Choice)



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