Effects of T and H on PS in L. latifolius Leaves

Overview

In many plant species, temperature and herbivory have been shown to affect photosynthetic rates and capacity (Zangerl et al. 2001, Pincebourde et al. 2017, Nabity et al. 2008). However, few studies have looked at the combined effects of temperature and herbivory on plant performance. Lupinus latifolius is a perennial plant native to the western coast of the United States. L. latifolius was chosen because it is a nitrogen-fixer, and it can facilitate the growth of other plant species in disturbed habitats (Halvorson et al. 1992). As such, observing how L. latifolius responds to future conditions we expect with climate change, specifically increased temperature and increased rates of herbivory, can provide insight about how plant communities will respond in the future. In this experiment, two growth chambers were used, and L. latifolius plants were placed in one of four treatment groups. The treatment groups are as follows: warmed and exposed to herbivory, warmed with no herbivory, unwarmed and exposed to herbivory, and unwarmed with no herbivory. For the herbivory treatments, a chewing insect will be used. MultispeQ, a portable plant-phenotyping instrument, will be used to capture photosynthetic parameters and relative chlorophyll. Certain morphological traits, such as plant height and LMA, will also be measured. L. latifolius has been shown to have a somewhat plastic response to temperature, meaning that the optimal temperature for photosynthesis may differ based on environmental conditions (Braatne and Bliss 1999). Due to potential phenotypic plasticity, the optimal temperatures within each temperature treatment will also be calculated using LI-COR (LI-6400XT). T tests and ANOVA will be conducted to determine whether differences between treatments are statistically significant. By conducting this research, we can gain a better understanding of how environmental and biotic factors associated with climate change can interact to affect plant performance, and affect plant community structure as a whole.

Protocols

Leaf Photosynthesis MultispeQ V1.0

Summary

Measures many photosynthesis-related parameters in <15 seconds, including:

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF
  • Relative Chlorophyll: SPAD
  • Proton Motive Force: ECSt, vH+, gH+
  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.
  • Leaf Thickness (in mm), angle, and cardinal direct...

Questions

  1. Treatment (Multiple Choice)
  2. Chamber (Multiple Choice)
  3. Plant ID (#) (Short Answer)
  4. Date collected (Short Answer)
  5. Position of leaf (Multiple Choice)
  6. Have the herbivores been introduced yet? (If applicable) (Multiple Choice)
  7. Time since last exposure to herbivores? (Short Answer)
  8. Level of leaf damage (Multiple Choice)
  9. Notes (Short Answer)

Locations

Team

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