Differences Tree Genera Leaf Changes at MSU, Fall2016


Fall 2016 Rationale Student research project studying the differences between different tree species on the north campus of MSU. These species are typically studied less and may be sampled by students in BS172. Studying Quercus shumardii, Malus *'Donald Wyman', Malus sutyzam and *Liquidambar.

Sampling Protocol 1. Select tree and its specific code.

  1. You will be using a step stool and stepping to the 2nd step do random sampling for 3 leaves then move stool & climb to 2nd step and do random sampling for 3 leaves; total of 6 leaves in a quadrant.

  2. Starting in the northeast quadrant, randomly select and measure 1 reachable dry, exterior leaf being sure to place the device at the leaf's natural angle. Also record the color of the leaf (leaves with multiple colors should have the most prevalent color first).

Using MultispeQ device #121 and phone #3 since people collecting data will be doing by themselves.

  1. Confirm the phi2 and SPAD III traces are correct before accepting the data.

  2. Measure total of 6 exterior leaves in this quadrant.

  3. Move to the southeast quadrant and record measurements on 6 randomly selected exterior leaves. Move to southwest quadrant and randomly measure 6 exterior leaves and rotate to the northwest quadrant and randomly measure 6 exterior leaves.

  4. Move to the next tree and repeat the process of using the step stool and moving from the northeast quadrant to the southeast, southwest and northwest (clockwise rotation around the tree).


Leaf Photosynthesis - MultispeQ Beta ONLY

Measures photosynthesis-related parameters in <15 seconds, including: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF, and SPAD. In addition, measures PAR (photosynthetically active radiation), ambient temperature and relative humidity.

Works with the MultispeQ Beta device only


  1. Which tree (genus & species)? (Multiple Choice)
  2. Which tree code? (Multiple Choice)
  3. Which quadrant? (Multiple Choice)
  4. What is leaf's color? (Multiple Choice)
  5. What is the leaf count? (Multiple Choice)



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