We've been measuring some plants in Growth Chamber before lights come on during predawn and would expect Phi2 approaching .85 at that time, instead the observations are all btw 0.7 and 0.5.
PAR is only being measured at 4-5 umol.
I think development of a protocol specifically for this FvFm of data is needed (and may already exist).
Generally, caution should be used with the Leaf Photosynthesis MultispeQ V1.0 protocol under these conditions.
Has any else seen this?
Not sure if ECS portion of trance is having an impact on PSII when dark adapted or if it is something else.