Measuring two population using phenotypic traits to determine genotypic traits in Syngenta maize lines.

Directions: - specify when plant was measured: morning: 8am - 11am mid-day: 11am - 2pm late-afternoon: 2pm - 5pm

  • measure 2nd leaf from the bottom
  • measure around the middle of the leaf


Leaf Photosynthesis MultispeQ V1.0 (development)

Change log


Updated absorbance blank and relative chlorophyll calibration intensities, which must also be updated in the absorbance section of this protocol.


Updated intensity of the "detector_read1" to 700 in accordance with the new IR LED calibration. This should output a correct baseline for phi2 and related photosynthesis data.


Changed PMF portion of trace from 300 pulses x 3 to 100 pulses x 3. Adjusted macro accordingly.


Measures photosynthesis-related parameters in <15 seconds, including: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF, and SPAD. In addition, absorbance measurements are taken at the following wavelengths - 420, 605, 650, 730, 850, 880, 950.

Also measures PAR (photosynthetically active radiation), ambient temperature/pressure/relative humidity, leaf thickness, leaf angle, leaf direction, and contactless leaf temperature.

The ECS auto-adjust feature tests the absorbance of the sample in the 525nm range (LED 1) before the measurement with 5 small pulses. Based on that value, it adjusts both the intensity of LED 1 (meas_intensity) and the size of the measurement pulse (pulsesize) during the actual measurement. These adjustments are based on testing on a series of thin, thick, green, and other colored leaves. The goal is to adjust the measuring pulse such that the detector values are in a high range (between 15k - 40k) regardless of leaf type.

This is the developm...


  1. What is the time of day? (Multiple Choice)
  2. Which greenhouse? (Multiple Choice)
  3. What is plant number? (Short Answer)




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