Measuring two population using phenotypic traits to determine genotypic traits in Syngenta maize lines.
Directions: - specify when plant was measured: morning: 8am - 11am mid-day: 11am - 2pm late-afternoon: 2pm - 5pm
- measure 2nd leaf from the bottom
- measure around the middle of the leaf
- Leaf Photosynthesis MultispeQ V1.0 (development)
Updated absorbance blank and relative chlorophyll calibration intensities, which must also be updated in the absorbance section of this protocol.
Updated intensity of the "detector_read1" to 700 in accordance with the new IR LED calibration. This should output a correct baseline for phi2 and related photosynthesis data.
Changed PMF portion of trace from 300 pulses x 3 to 100 pulses x 3. Adjusted macro accordingly.
Measures photosynthesis-related parameters in <15 seconds, including: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF, and SPAD. In addition, absorbance measurements are taken at the following wavelengths - 420, 605, 650, 730, 850, 880, 950.
Also measures PAR (photosynthetically active radiation), ambient temperature/pressure/relative humidity, leaf thickness, leaf angle, leaf direction, and contactless leaf temperature.
The ECS auto-adjust feature tests the absorbance of the sample in the 525nm range (LED 1) before the measurement with 5 small pulses. Based on that value, it adjusts both the intensity of LED 1 (meas_intensity) and the size of the measurement pulse (pulsesize) during the actual measurement. These adjustments are based on testing on a series of thin, thick, green, and other colored leaves. The goal is to adjust the measuring pulse such that the detector values are in a high range (between 15k - 40k) regardless of leaf type.
This is the developm...
- What is the time of day? (Multiple Choice)
- Which greenhouse? (Multiple Choice)
- What is plant number? (Short Answer)