The purpose of this experiment is to determine the difference(s) between phiII, NPQt, LEF and chlorophyll content on the different position of Quercus bicolor leaves.
We want to know, How does the position of the leaves in respect with cardinal direction of 4 quadrants; 1 being the northeast, 2 being the southeast, 3 being the southwest and 4 being the northwest on the tree affect photosynthesis efficiency and chlorophyll content in the Quercus bicolor?
We also also want to see how our data compares to the similar study in the fall of 2014.
The reason for these questions are to apply it to a real life application of planting trees, to have the most efficient photosynthesis to produce the most fruit.
We hypothesize that the photosynthetic efficiency and chlorophyll content will be higher on the east side (quadrants 1 and 2) of the tree compared to the west side (quadrants 3 and 4) because of the brightness of the sun is more during sunrise from morning to mid-day then it is during sunset from mid-day to dusk. Because of the location of other trees and buildings there is going to be more shade in the afternoon on the 3 trees compared to the morning, which may have an effect on the photosynthetic efficiency of the cardinal direction directly related to where the sun position is, resulting in a higher value of photosynthesis on the east face of the tree compared to west face.
This study is a local study for BS 172 members to take part in. Checking out the multispeQ #124 device and android tablet #6 for 2 hour increments is how the measurements will be made for this research. The Quercus bicolor; 20050351 07, 20050351 08, 20080316 * 02 are the organisms for this study. These trees were planted in 2005, 2005, and 2008 respectively The sample size is the 3 trees There is 10 measurements per quadrant resulting in 120 total measurements across the 3 trees. We will be using the following protocols: The One Protocol II, to measure PhiII, LEF and light intensity. SPADIII, to measure chlorophyll content. This is because we are comparing our data with the previous year. Methods
Sync MultispeQ device #124 with tablet #6 Turn off wifi on your tablet/phone before going outside. When you get to the tree turn on your MultispeQ and make sure it is synced with your tablet/phone. Find the correct tree that matches the correct tree code on the app. Split tree into 4 quadrants. (quadrant 1 = Northeast, quadrant 2=Southeast, quadrant 3=Southwest, quadrant 4=Northwest) Randomly pick an outside leaf by just selecting a leaf from the left to right in each quadrant, starting with quadrant 1, and answer all questions according to the leaf you have selected. If the leaf you have chosen has multiple colors, the 1st color is for the most prominent one while the 2nd is for the less prominent. If there are 3 or more colors then select that option. The questions include: What tree code? (2008031602,2005035108,20050351*07), What quadrant? (1-Northeast,2-Southeast,3-Southwest,4-Northwest), What position of the leaf? (Middle,Bottom), What color is the leaf? (dark green,green,green-yellow,yellow-green,yellow,rust-yellow,brown-yellow,yellow-brown,brown,green-orange,yellow-orange,orange-yellow,red-green,green-red,yellow-red,red-yellow,3 or more), What # leaf? (1,2,3,4,5,6,7,8,9,10). Attach your multispeQ to the TOP of a DRY leaf without disrupting the natural bend of the leaf. Make sure the white circular light detector is pointed UP and not blocked. Once you clamp the multispeQ completely covering the two lens with the leaf, IMMEDIATELY push "take the measurement" on your tablet/phone. When you see the graphs check to make sure they look proper then you may remove the multispeQ from the leaf if they do. Confirm that the data is within expected ranges, which is NPQT and PhiII must be positive. If it is, keep it, if not, discard it. Click "Okay" to save the data. Repeat steps 7 through 11, nine more times in quadrant 1. Then, 10 times in each of the other 3 quadrants while changing the quadrant you're in on your tablet/phone. Repeat steps 5 through 12 for the next two trees. To upload the data, reconnect with wifi and refresh the "list of projects" page. You will be notified if the data was uploaded. Run a One-way ANOVA or Two-way ANOVA test to compare the different quadrants between each tree. This will also be used to compare data from 2014 and 2015.
- The One v3.0 (Phi2, NPQt, using Multi-Phase Flash)
The One Protocol to rule them all! Phi2, NPQt combined.
Changes from previous The One protocol: - Removed the Phi1 measurement, which was producing a lot of invalid data (removed by Dan and Frank) - Implemented the multi-phase flash protocol created by Tom Avenson
- Chlorophyll content (SPAD) III
REQUIREMENTS: Firmware v0.41 or higher. Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness. Improvements over Chlorophyll content (SPAD): - performs 3 different spads at 3 intensities for thin and thick leaves - auto-chooses appropriate intensity - is calibrated to a Minolta SPAD device from factory.
Improvements over previous version -
- Compatible with firmware v0.41 or higher.
- What tree code?
- What is position of the leaf?
- What is the color of the leaf?
- What # leaf?
- What quadrant?