Dry Beans Root Rot Resistance

Overview

We are testing over 50 different varieties of beans, how resistant they are to the presence of disease and if these diseases could later be predicted in fields.

Each bean variety was exposed to two different disease agents and a control. Fusarium brasiliense, a disease agent of Sudden Soybean Death Syndrome. The other disease was Rhizoctonia solani AG2-2 which brings a range of negative impacts on the bean including damping off of seedlings, cankers in grown plants and root dieback. All can lead to lower yields.

Bean varieties were exposed to each of these agents once per replicate. Each replicate had a total of 150 unique plots per replicate. A plot is approximately 10 feet long and contains only a single row of the bean crop. There are 4 replicates in the field.

The results of this experiment have potential to help predict disease presence at an early onset through analysis of photosynthetic data. It will also help further our understanding of which varieties of beans have resistance to the diseases.

Protocols

Leaf Photosynthesis MultispeQ V1.0

Summary

Measures many photosynthesis-related parameters in <15 seconds, including:

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF
  • Relative Chlorophyll: SPAD
  • Proton Motive Force: ECSt, vH+, gH+
  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.
  • Leaf Thickness (in mm), angle, and cardinal direction
  • Leaf Temperature and differential from ambient temperature
  • Environmental conditions: PAR and ambient temperature/pressure/humidity

Description

Leaf Photosynthesis MultispeQ V1.0 is the classic and by far the most utilized PhotosynQ Protocol.

This protocol delivers a wide range of plant photosynthetic parameters, a full list can be found here

If you want to see the exact calculations for each parameter, please see the associated macro called "Leaf Photosynthesis MultispeQ V1.0".

There are two publications about the MultispeQ Beta device covering many of parameters and their comparisons to commercial instruments. While the beta was an older model device, the calculations and comparisons are still true for this protocol and V1.0 device. You can find them at the Royal Society for Open Science, and Plant, Cell & Environmental Journal.

The Leaf Photosynthesis protocol collects many different measurements from a leaf as quickly as possib...

Questions

  1. Genotype (Multiple Choice)
  2. Treatment (Multiple Choice)
  3. leaf position (Multiple Choice)
  4. REP (Multiple Choice)
  5. device number (Short Answer)
  6. growth stage (Short Answer)

Locations

Discussions

Team

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