Dry Beans Root Rot Resistance

Overview

We are testing over 50 different varieties of beans, how resistant they are to the presence of disease and if these diseases could later be predicted in fields.

Each bean variety was exposed to two different disease agents and a control. Fusarium brasiliense, a disease agent of Sudden Soybean Death Syndrome. The other disease was Rhizoctonia solani AG2-2 which brings a range of negative impacts on the bean including damping off of seedlings, cankers in grown plants and root dieback. All can lead to lower yields.

Bean varieties were exposed to each of these agents once per replicate. Each replicate had a total of 150 unique plots per replicate. A plot is approximately 10 feet long and contains only a single row of the bean crop. There are 4 replicates in the field.

The results of this experiment have potential to help predict disease presence at an early onset through analysis of photosynthetic data. It will also help further our understanding of which varieties of beans have resistance to the diseases.

Protocols

Leaf Photosynthesis MultispeQ V1.0

Summary

Measures many photosynthesis-related parameters in <15 seconds, including:

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF
  • Relative Chlorophyll: SPAD
  • Proton Motive Force: ECSt, vH+, gH+
  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.
  • Leaf Thickness (in mm), angle, and cardinal direct...

Questions

  1. Genotype (Multiple Choice)
  2. Treatment (Multiple Choice)
  3. leaf position (Multiple Choice)
  4. REP (Multiple Choice)
  5. device number (Short Answer)
  6. growth stage (Short Answer)

Locations

Team

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