Chlamy Group BS 172 Summer 2015

Overview

In this project we will be studying the photosynthetic efficiency of Chlamydomonas under different periods of light and dark. We have chosen the to experiment with Chlamydomonas because of the quick pace of its replication and the ease with which it can be cultured in lab. This experiment is being done in order to provide insight into the sensitivity of Chlamydomonas to light exposure. As a group, it is predicted that Chlamydomonas will react differently toward varying light conditions. We feel that the treatment with the highest amount of light exposure will have an optimal photosynthetic efficiency and that the treatment with the least amount of light will be affected negatively in comparison to the other treatments. We will have a condition in 24 hours light, 12 hours light 12 hours dark, 6 hours light 18 hours dark, and 24 hours dark.  We will also have an additional condition exposed to 24 hours light placed under a petri dish of water to simulate the light experienced by Chlamy floating below the water's surface.  We will setup our samples on Monday, take initial measurements of optical density, photosynthetic efficiency, and chlorophyll content.  Additional measurements will be taken 24 hours later, 48 hours later and 72 hours later.

Protocols

The One Protocol (Phi2, PSI, NPQ) II

The One Protocol to rule them all! Phi2, PSI, NPQt, combined!

Changes from previous The One protocol: - no longer outputs signal from the far red period, which simplifies the signal output for the user - changed distance between measuring pulses from 10,000 to 2,000 which improves measurement resolution - has an initial stabilization peri...

Chlorophyll content (SPAD) I

REQUIREMENTS: Firmware v0.38 or lower (used for older devices which do not have a SPAD calibration, generally device ID 87 and lower).Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness.

Questions

  1. Condition (Multiple Choice)
  2. Petri Dish Number (Multiple Choice)
  3. Day (Multiple Choice)

Team

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Project Lead (62)
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Collaborator (90)
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