WASP_test_850_1_series


This protocol measures a series of factors that allow us to estimate: stomatal conductance (via changes in humidity and leaf temperature);
ATP synthase conductivity, pmf parameters through DIRK(ECS) The rate of turnover of the cytochrome b6f complex through DIRKP(700) Chlorophyll fluorescence parameters Phi2, NPQ(t), PhiNPQ, PhiNO, qL(t) Saturation pulse estimation of PSI parameters (Y(I), PSI(ox), PSI(or)).

[
  {
    "_protocol_set_": [
      {
        "label": "wasp=10 int=1000",
        "set_led_delay": [
          [
            2,
            1000,
            0
          ]
        ],
        "protocols_delay": 10,
        "autogain": [
          [
            1,
            2,
            3,
            5,
            50000
          ],
          [
            2,
            8,
            1,
            10,
            50000
          ]
        ],
        "pulses": [
          100,
          100,
          100,
          100
        ],
        "pulse_distance": [
          3000,
          3000,
          3000,
          3000
        ],
        "pulse_length": [
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ],
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ],
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ],
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ]
        ],
        "pulsed_lights": [
          [
            8,
            8,
            8,
            8
          ],
          [
            8,
            8,
            8,
            8
          ],
          [
            8,
            8,
            8,
            8
          ],
          [
            8,
            8,
            8,
            8
          ]
        ],
        "suppress_m_pulse": [
          [
            0,
            0,
            1,
            0
          ],
          [
            0,
            0,
            1,
            0
          ],
          [
            0,
            0,
            1,
            0
          ],
          [
            0,
            0,
            1,
            0
          ]
        ],
        "detectors": [
          [
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1
          ]
        ],
        "pulsed_lights_brightness": [
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ],
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ],
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ],
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ]
        ],
        "nonpulsed_lights": [
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ]
        ],
        "nonpulsed_lights_brightness": [
          [
            0
          ],
          [
            400
          ],
          [
            8000
          ],
          [
            0
          ]
        ],
        "wasp_lights_brightness": [
          [
            1000,
            1000,
            1000,
            1000
          ],
          [
            1000,
            1000,
            1000,
            1000
          ],
          [
            1000,
            1000,
            1000,
            1000
          ],
          [
            1000,
            1000,
            1000,
            1000
          ]
        ],
        "wasp_lights": [
          [
            0,
            7,
            7,
            0
          ],
          [
            0,
            7,
            7,
            0
          ],
          [
            0,
            7,
            7,
            0
          ],
          [
            0,
            7,
            7,
            0
          ]
        ],
        "environmental": [
          [
            "light_intensity"
          ],
          [
            "temperature_humidity_pressure"
          ],
          [
            "temperature_humidity_pressure2"
          ],
          [
            "contactless_temp"
          ],
          [
            "thickness"
          ]
        ],
        "averages": 1
      },
      {
        "label": "wasp=10us int=500",
        "set_led_delay": [
          [
            2,
            1000,
            0
          ]
        ],
        "protocols_delay": 10,
        "autogain": [
          [
            1,
            2,
            3,
            5,
            50000
          ],
          [
            2,
            8,
            1,
            10,
            50000
          ]
        ],
        "pulses": [
          100,
          100,
          100,
          100
        ],
        "pulse_distance": [
          3000,
          3000,
          3000,
          3000
        ],
        "pulse_length": [
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ],
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ],
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ],
          [
            "auto_duration2",
            "auto_duration2",
            "auto_duration2",
            "auto_duration2"
          ]
        ],
        "pulsed_lights": [
          [
            8,
            8,
            8,
            8
          ],
          [
            8,
            8,
            8,
            8
          ],
          [
            8,
            8,
            8,
            8
          ],
          [
            8,
            8,
            8,
            8
          ]
        ],
        "suppress_m_pulse": [
          [
            0,
            0,
            1,
            0
          ],
          [
            0,
            0,
            1,
            0
          ],
          [
            0,
            0,
            1,
            0
          ],
          [
            0,
            0,
            1,
            0
          ]
        ],
        "detectors": [
          [
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1
          ]
        ],
        "pulsed_lights_brightness": [
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ],
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ],
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ],
          [
            "auto_bright2",
            "auto_bright2",
            "auto_bright2",
            "auto_bright2"
          ]
        ],
        "nonpulsed_lights": [
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ]
        ],
        "nonpulsed_lights_brightness": [
          [
            0
          ],
          [
            400
          ],
          [
            8000
          ],
          [
            0
          ]
        ],
        "wasp_lights_brightness": [
          [
            500,
            500,
            500,
            500
          ],
          [
            500,
            500,
            500,
            500
          ],
          [
            500,
            500,
            500,
            500
          ],
          [
            500,
            500,
            500,
            500
          ]
        ],
        "wasp_lights": [
          [
            0,
            7,
            7,
            0
          ],
          [
            0,
            7,
            7,
            0
          ],
          [
            0,
            7,
            7,
            0
          ],
          [
            0,
            7,
            7,
            0
          ]
        ],
        "environmental": [
          [
            "light_intensity"
          ],
          [
            "temperature_humidity_pressure"
          ],
          [
            "temperature_humidity_pressure2"
          ],
          [
            "contactless_temp"
          ],
          [
            "thickness"
          ]
        ],
        "averages": 1
      }
    ]
  }
]
Dave in cap img 4615
Created by

David M. Kramer


Category

Plants
Connected Macro

wasp_850_test_1


Compatible Instruments

MultispeQ v1.0 MultispeQ v2.0


Latest Update

Jul 2017