The One Protocol (Phi2, PSI, NPQ) II


The One Protocol to rule them all! Phi2, PSI, NPQt, combined!

Changes from previous The One protocol: - no longer outputs signal from the far red period, which simplifies the signal output for the user - changed distance between measuring pulses from 10,000 to 2,000 which improves measurement resolution - has an initial stabilization period, so Fs has a chance to adjust to the actinic. - the measuring lights are used only when actually needed (before lights 15 and 10 were always pulsed, even when the values were not used in the macro outputs) - removed light 2 (650) on add-on board during the actinic periods so now actinic is just light 20 (650) on the main boards. This was causing the actinic to not match ambient, as the combination of two 650 lights was greater than the ambient value.

[
  {
    "pulses": [
      200,
      20,
      50,
      100,
      200,
      4000,
      300,
      200,
      100
    ],
    "get_ir_baseline": [
      15,
      14
    ],
    "environmental": [
      [
        "relative_humidity",
        0
      ],
      [
        "temperature",
        0
      ],
      [
        "light_intensity",
        0
      ]
    ],
    "act1_lights": [
      20,
      20,
      20,
      20,
      0,
      0,
      0,
      20,
      20
    ],
    "act2_lights": [
      0,
      0,
      0,
      2,
      0,
      11,
      0,
      2,
      0
    ],
    "tcs_to_act": 100,
    "act_background_light": 20,
    "act_intensities": [
      -1,
      -1,
      -1,
      400,
      0,
      0,
      0,
      400,
      -1
    ],
    "cal_intensities": [
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095
    ],
    "meas_intensities": [
      4095,
      0,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095
    ],
    "pulsedistance": 2000,
    "pulsesize": 10,
    "detectors": [
      [
        34
      ],
      [
        34
      ],
      [
        34,
        34
      ],
      [
        34,
        34
      ],
      [
        34,
        34
      ],
      [
        34
      ],
      [
        34,
        34
      ],
      [
        34
      ],
      [
        34
      ]
    ],
    "meas_lights": [
      [
        0
      ],
      [
        14
      ],
      [
        10,
        15
      ],
      [
        10,
        15
      ],
      [
        10,
        15
      ],
      [
        0
      ],
      [
        10,
        15
      ],
      [
        10
      ],
      [
        10
      ]
    ]
  }
]

I would like to use this protocol using MulitispeQ V 1.0. I searched it and click on this protocol and start measurement. But result is only one value of light intensity. Probably, the protocol is not completely imported in my mobile. Let me know any source from where I get step by step info to use protocols designed by PhotosynQ community

Habib-ur-Rehman Athar Bahauddin Zakariya University, Multan 60800, Pakistan

012409 pbi 160 low low res headshot
Created by

Greg Austic


Category

Plants

Compatible Instruments

MultispeQ beta


Latest Update

Jul 2016