s_WASP_test_short_pulse


This protocol measures a series of factors that allow us to estimate: stomatal conductance (via changes in humidity and leaf temperature);
ATP synthase conductivity, pmf parameters through DIRK(ECS) The rate of turnover of the cytochrome b6f complex through DIRKP(700) Chlorophyll fluorescence parameters Phi2, NPQ(t), PhiNPQ, PhiNO, qL(t) Saturation pulse estimation of PSI parameters (Y(I), PSI(ox), PSI(or)).

[
  {
    "v_arrays": [
      [
        0,
        5,
        5,
        5,
        10,
        20,
        40,
        80,
        160,
        320,
        640,
        1280,
        80,
        160,
        80,
        160,
        5,
        5
      ]
    ],
    "set_repeats": 1,
    "_protocol_set_": [
      {
        "label": "wasp",
        "averages_delay": 10000,
        "pulses": [
          30,
          1,
          100
        ],
        "pulse_distance": [
          100000,
          1000,
          100000
        ],
        "pulse_length": [
          [
            3,
            3,
            3,
            3,
            3
          ],
          [
            3,
            3,
            3,
            3,
            3
          ],
          [
            3,
            3,
            3,
            3,
            3
          ]
        ],
        "pulsed_lights": [
          [
            3,
            3,
            3,
            3,
            3
          ],
          [
            3,
            3,
            3,
            3,
            3
          ],
          [
            3,
            3,
            3,
            3,
            3
          ]
        ],
        "suppress_m_pulse": [
          [
            0,
            0,
            0,
            0,
            0
          ],
          [
            0,
            0,
            0,
            0,
            0
          ],
          [
            0,
            0,
            0,
            0,
            0
          ]
        ],
        "detectors": [
          [
            1,
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1,
            1
          ],
          [
            1,
            1,
            1,
            1,
            1
          ]
        ],
        "pulsed_lights_brightness": [
          [
            300,
            300,
            300,
            300,
            300
          ],
          [
            300,
            300,
            300,
            300,
            300
          ],
          [
            300,
            300,
            300,
            300,
            300
          ]
        ],
        "nonpulsed_lights": [
          [
            2
          ],
          [
            2
          ],
          [
            2
          ]
        ],
        "nonpulsed_lights_brightness": [
          [
            0
          ],
          [
            1000
          ],
          [
            0
          ]
        ],
        "wasp_lights_brightness": [
          [
            2000,
            2000,
            2000,
            2000,
            0
          ],
          [
            2000,
            2000,
            2000,
            2000,
            0
          ],
          [
            2000,
            2000,
            2000,
            2000,
            0
          ]
        ],
        "wasp_lights": [
          [
            6,
            10,
            8,
            9,
            10
          ],
          [
            6,
            10,
            8,
            9,
            10
          ],
          [
            6,
            10,
            8,
            9,
            10
          ]
        ],
        "wasp_delay": [
          [
            1500,
            1500,
            1500,
            1500,
            1500
          ],
          [
            1500,
            1500,
            1500,
            1500,
            1500
          ],
          [
            1500,
            1500,
            1500,
            1500,
            1500
          ]
        ],
        "environmental": [

        ],
        "averages": 1,
        "protocol_repeats": 1
      },
      {
        "label": "abs",
        "protocols_delay": 10,
        "pulses": [
          10
        ],
        "pulse_distance": [
          30000
        ],
        "pulse_length": [
          [
            20,
            20,
            20,
            20,
            20
          ]
        ],
        "pulsed_lights": [
          [
            6,
            10,
            8,
            9,
            10
          ]
        ],
        "detectors": [
          [
            1,
            1,
            1,
            1,
            1
          ]
        ],
        "pulsed_lights_brightness": [
          [
            1000,
            1000,
            1000,
            1000,
            0
          ]
        ],
        "nonpulsed_lights": [
          [
            2
          ],
          [
            2
          ],
          [
            2
          ]
        ],
        "nonpulsed_lights_brightness": [
          [
            0
          ]
        ],
        "environmental": [

        ],
        "averages": 10
      }
    ]
  }
]
Dave in cap img 4615
Created by

David M. Kramer


Category

Algae
Connected Macro

wasp_test_m_v


Compatible Instruments

MultispeQ v1.0 MultispeQ v2.0


Latest Update

Nov 2018