This protocol measures a series of factors that allow us to estimate:
stomatal conductance (via changes in humidity and leaf temperature);
ATP synthase conductivity, pmf parameters through DIRK(ECS)
The rate of turnover of the cytochrome b6f complex through DIRKP(700)
Chlorophyll fluorescence parameters Phi2, NPQ(t), PhiNPQ, PhiNO, qL(t)
Saturation pulse estimation of PSI parameters (Y(I), PSI(ox), PSI(or)).
[
{
"_protocol_set_": [
{
"label": "no_leaf_baseline",
"start_on_open": 2,
"energy_save_timeout": 300000,
"energy_min_wake_time": 7000,
"set_light_intensity": 1000,
"environmental": [
[
"previous_light_intensity"
],
[
"temperature_humidity_pressure"
],
[
"temperature_humidity_pressure2"
],
[
"contactless_temp"
],
[
"compass_and_angle"
]
],
"averages": 1
},
{
"label": "DIRK_ECS",
"par_led_start_on_close": 2,
"protocols_delay": 10,
"autogain": [
[
2,
1,
3,
12,
50000
],
[
3,
8,
1,
80,
50000
]
],
"environmental": [
[
"previous_light_intensity"
],
[
"temperature_humidity_pressure"
],
[
"temperature_humidity_pressure2"
],
[
"contactless_temp"
],
[
"thickness"
]
],
"averages": 1,
"pulse_distance": [
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500
],
"pulse_length": [
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
],
[
"auto_duration2"
]
],
"nonpulsed_lights_brightness": [
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
]
],
"pulsed_lights": [
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
3
],
[
3
],
[
3
]
],
"detectors": [
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
]
],
"pulsed_lights_brightness": [
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
],
[
"auto_bright2"
]
],
"pulses": [
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
20,
20,
100
],
"nonpulsed_lights": [
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
]
]
},
{
"label": "DIRK_P700",
"environmental": [
[
"previous_light_intensity"
],
[
"temperature_humidity_pressure"
],
[
"temperature_humidity_pressure2"
],
[
"contactless_temp"
],
[
"compass_and_angle"
]
],
"protocols_delay": 1,
"averages": 1,
"pulses": [
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100
],
"pulse_distance": [
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500
],
"pulse_length": [
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
],
[
"auto_duration3"
]
],
"nonpulsed_lights_brightness": [
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
]
],
"pulsed_lights": [
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
]
],
"detectors": [
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
]
],
"pulsed_lights_brightness": [
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
],
[
"auto_bright3"
]
],
"nonpulsed_lights": [
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
]
]
},
{
"label": "PAM",
"pulses": [
100,
30,
15,
15,
15,
15,
15,
600,
15,
30,
15,
15,
15,
15
],
"nonpulsed_lights": [
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
9
],
[
0
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
]
],
"nonpulsed_lights_brightness": [
[
"light_intensity"
],
[
8000
],
[
7000
],
[
6000
],
[
5000
],
[
4000
],
[
0
],
[
2090
],
[
0
],
[
8000
],
[
6000
],
[
4000
],
[
3000
],
[
"light_intensity"
]
],
"pulse_distance": [
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000
],
"pulsed_lights_brightness": [
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
0
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
],
[
400,
"auto_bright3"
]
],
"pulse_length": [
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
0
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
],
[
30,
"auto_duration3"
]
],
"detectors": [
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
0
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
]
],
"pulsed_lights": [
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
0
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
]
],
"environmental": [
[
"previous_light_intensity"
],
[
"temperature_humidity_pressure"
],
[
"temperature_humidity_pressure2"
],
[
"contactless_temp"
],
[
"thickness"
],
[
"compass_and_angle"
]
],
"averages": 1
},
{
"label": "SPAD",
"pulses": [
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10
],
"nonpulsed_lights": [
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
]
],
"nonpulsed_lights_brightness": [
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
]
],
"pulse_distance": [
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000
],
"pulsed_lights_brightness": [
[
30
],
[
450
],
[
75
],
[
80
],
[
700
],
[
700
],
[
700
],
[
700
],
[
375
],
[
3000
],
[
500
],
[
500
],
[
2250
],
[
2250
],
[
2250
],
[
2250
],
[
1800
],
[
7000
],
[
1800
],
[
2000
],
[
2250
],
[
2250
],
[
2250
],
[
2250
]
],
"pulse_length": [
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
]
],
"detectors": [
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
],
[
1
],
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
],
[
1
],
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
],
[
1
]
],
"pulsed_lights": [
[
1
],
[
2
],
[
3
],
[
4
],
[
6
],
[
8
],
[
9
],
[
10
],
[
1
],
[
2
],
[
3
],
[
4
],
[
6
],
[
8
],
[
9
],
[
10
],
[
1
],
[
2
],
[
3
],
[
4
],
[
6
],
[
8
],
[
9
],
[
10
]
],
"recall": [
"colorcal_blank1[1]",
"colorcal_blank1[2]",
"colorcal_blank1[3]",
"colorcal_blank1[4]",
"colorcal_blank1[6]",
"colorcal_blank1[8]",
"colorcal_blank1[9]",
"colorcal_blank1[10]",
"colorcal_blank2[1]",
"colorcal_blank2[2]",
"colorcal_blank2[3]",
"colorcal_blank2[4]",
"colorcal_blank2[6]",
"colorcal_blank2[8]",
"colorcal_blank2[9]",
"colorcal_blank2[10]",
"colorcal_blank3[1]",
"colorcal_blank3[2]",
"colorcal_blank3[3]",
"colorcal_blank3[4]",
"colorcal_blank3[6]",
"colorcal_blank3[8]",
"colorcal_blank3[9]",
"colorcal_blank3[10]",
"colorcal_intensity1_slope[2]",
"colorcal_intensity1_yint[2]",
"colorcal_intensity2_slope[2]",
"colorcal_intensity2_yint[2]",
"colorcal_intensity3_slope[2]",
"colorcal_intensity3_yint[2]",
"ir_baseline_slope[5]",
"ir_baseline_yint[5]",
"ir_baseline_slope[3]",
"ir_baseline_yint[3]"
],
"environmental": [
[
"detector_read1",
5,
700,
1,
30
],
[
"detector_read2",
1,
150,
3,
15
],
[
"light_intensity"
],
[
"temperature_humidity_pressure"
],
[
"temperature_humidity_pressure2"
],
[
"contactless_temp"
],
[
"thickness"
],
[
"compass_and_angle"
]
],
"averages": 1
}
]
}
]
Created by
David M. Kramer
Category
Algae
Connected Macro
Compatible Instruments
MultispeQ v1.0 MultispeQ v2.0
Latest Update
Feb 2018
