This protocol measures a series of factors that allow us to estimate:
stomatal conductance (via changes in humidity and leaf temperature);
ATP synthase conductivity, pmf parameters through DIRK(ECS)
The rate of turnover of the cytochrome b6f complex through DIRKP(700)
Chlorophyll fluorescence parameters Phi2, NPQ(t), PhiNPQ, PhiNO, qL(t)
Saturation pulse estimation of PSI parameters (Y(I), PSI(ox), PSI(or)).
[
{
"_protocol_set_": [
{
"energy_save_timeout": 300000,
"energy_min_wake_time": 7000,
"set_light_intensity": 500,
"environmental": [
[
"p_light"
],
[
"thp"
],
[
"thp2"
],
[
"contactless_temp"
]
],
"averages": 1
},
{
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[
2,
5000,
250
],
[
2,
5000,
500
]
],
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[
"p_light"
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[
"thp"
],
[
"thp2"
],
[
"contactless_temp"
],
[
"thickness"
]
],
"averages": 1
},
{
"protocols_delay": 10,
"autogain": [
[
2,
1,
3,
20,
50000
],
[
3,
8,
1,
80,
50000
]
],
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[
"p_light"
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[
"thp"
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[
"thp2"
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[
"contactless_temp"
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[
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]
],
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"pulse_distance": [
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500
],
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[
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[
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[
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[
"a_d2"
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[
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[
"a_d2"
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[
"a_d2"
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[
"a_d2"
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[
"a_d2"
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[
"a_d2"
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[
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[
"a_d2"
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[
"a_d2"
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[
"a_d2"
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[
"a_d2"
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[
"a_d2"
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[
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[
"a_d2"
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[
"a_d2"
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],
"nonpulsed_lights_brightness": [
[
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[
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[
0
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[
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[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
],
[
"light_intensity"
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],
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
3
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[
3
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[
3
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3
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[
3
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[
3
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3
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3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
3
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[
1
],
[
1
],
[
1
]
],
"pulsed_lights_brightness": [
[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
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[
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[
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[
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
],
[
"a_b2"
],
[
"a_b2"
],
[
"a_b2"
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[
"a_b2"
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[
"a_b2"
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[
"a_b2"
]
],
"pulses": [
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
20,
20,
100
],
"nonpulsed_lights": [
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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2
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[
2
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[
2
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[
2
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[
2
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[
2
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[
2
]
]
},
{
"environmental": [
[
"p_light"
],
[
"thp"
],
[
"thp2"
],
[
"contactless_temp"
]
],
"protocols_delay": 1,
"averages": 1,
"pulses": [
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100,
100,
20,
100
],
"pulse_distance": [
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500,
1500
],
"pulse_length": [
[
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
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[
"a_d3"
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[
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[
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[
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
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[
"a_d3"
]
],
"nonpulsed_lights_brightness": [
[
"light_intensity"
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[
"light_intensity"
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[
0
],
[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
],
[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
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[
"light_intensity"
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[
0
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[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
],
[
"light_intensity"
],
[
0
],
[
"light_intensity"
]
],
"pulsed_lights": [
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
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[
8
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[
8
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[
8
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[
8
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[
8
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[
8
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[
8
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[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
],
[
8
]
],
"detectors": [
[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
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[
1
],
[
1
],
[
1
],
[
1
],
[
1
],
[
1
]
],
"pulsed_lights_brightness": [
[
"a_b3"
],
[
"a_b3"
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[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
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[
"a_b3"
],
[
"a_b3"
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[
"a_b3"
],
[
"a_b3"
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[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
],
[
"a_b3"
]
],
"nonpulsed_lights": [
[
2
],
[
2
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[
2
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[
2
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[
2
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[
2
],
[
2
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[
2
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[
2
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[
2
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[
2
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[
2
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[
2
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[
2
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[
2
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[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
]
]
},
{
"pulses": [
100,
30,
15,
15,
15,
15,
15,
600,
15,
30,
15,
15,
15,
15
],
"nonpulsed_lights": [
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
],
[
9
],
[
0
],
[
2
],
[
2
],
[
2
],
[
2
],
[
2
]
],
"nonpulsed_lights_brightness": [
[
"light_intensity"
],
[
8000
],
[
7000
],
[
6000
],
[
5000
],
[
4000
],
[
0
],
[
2090
],
[
0
],
[
8000
],
[
6000
],
[
4000
],
[
3000
],
[
"light_intensity"
]
],
"pulse_distance": [
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000,
5000
],
"pulsed_lights_brightness": [
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
0
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
],
[
400,
"a_b3"
]
],
"pulse_length": [
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
0
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
],
[
30,
"a_d3"
]
],
"detectors": [
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
0
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
],
[
1,
1
]
],
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[
3,
8
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[
3,
8
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[
3,
8
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[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
0,
0
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
],
[
3,
8
]
],
"environmental": [
[
"p_light"
],
[
"thp"
],
[
"thp2"
],
[
"contactless_temp"
],
[
"thickness"
]
],
"averages": 1
},
{
"environmental": [
[
"p_light"
],
[
"thp"
],
[
"thp2"
],
[
"contactless_temp"
],
[
"thickness"
],
[
"compass_and_angle"
]
],
"averages": 1,
"protocols": 3,
"protocols_delay": 300
},
{
"pulses": [
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10,
10
],
"nonpulsed_lights": [
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
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0
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[
0
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0
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0
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[
0
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0
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0
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0
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0
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0
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[
0
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[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
]
],
"nonpulsed_lights_brightness": [
[
0
],
[
0
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[
0
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[
0
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[
0
],
[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
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[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
],
[
0
]
],
"pulse_distance": [
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000,
1000
],
"pulsed_lights_brightness": [
[
30
],
[
450
],
[
75
],
[
80
],
[
700
],
[
700
],
[
700
],
[
700
],
[
375
],
[
3000
],
[
500
],
[
500
],
[
2250
],
[
2250
],
[
2250
],
[
2250
],
[
1800
],
[
7000
],
[
1800
],
[
2000
],
[
2250
],
[
2250
],
[
2250
],
[
2250
]
],
"pulse_length": [
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
],
[
20
]
],
"detectors": [
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
],
[
1
],
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
],
[
1
],
[
3
],
[
3
],
[
3
],
[
3
],
[
1
],
[
1
],
[
1
],
[
1
]
],
"pulsed_lights": [
[
1
],
[
2
],
[
3
],
[
4
],
[
6
],
[
8
],
[
9
],
[
10
],
[
1
],
[
2
],
[
3
],
[
4
],
[
6
],
[
8
],
[
9
],
[
10
],
[
1
],
[
2
],
[
3
],
[
4
],
[
6
],
[
8
],
[
9
],
[
10
]
],
"environmental": [
[
"detector_read1",
5,
700,
1,
30
],
[
"detector_read2",
1,
150,
3,
15
],
[
"p_light"
],
[
"thp"
],
[
"thp2"
],
[
"contactless_temp"
],
[
"thickness"
]
],
"averages": 1
}
]
}
]
Created by
David M. Kramer
Category
Algae
Connected Macro
Compatible Instruments
MultispeQ v1.0 MultispeQ v2.0
Latest Update
Aug 2017
