Phi2 FIX ACTINIC 250 - RG - WITHOUT FR + final


This protocol allow you to follow changes on Phi2 over time with fixed actinic light set at 250 uE. The total time of the protocol is about 1 minute. Difference from the previous protocols is that this one exclude the final far-red oxidation allowing to observe the F0' increase after illumination. This will be useful to asses antimycin A effect.

[
  {
    "pulses": [
      10,
      20,
      20,
      50,
      500,
      50,
      500,
      50,
      500,
      50,
      500,
      50,
      500,
      50,
      500,
      50,
      500,
      50,
      500,
      50,
      2000
    ],
    "get_ir_baseline": [
      15,
      14
    ],
    "act_intensities": [
      0,
      0,
      0,
      800,
      68,
      800,
      68,
      800,
      68,
      800,
      68,
      800,
      68,
      800,
      68,
      800,
      68,
      800,
      68,
      800,
      0
    ],
    "act1_lights": [
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20,
      20
    ],
    "meas_intensities": [
      0,
      0,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095,
      4095
    ],
    "cal_intensities": [
      0,
      4095,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0,
      0
    ],
    "pulsedistance": 10000,
    "pulsesize": 10,
    "detectors": [
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ],
      [
        34
      ]
    ],
    "meas_lights": [
      [
        0
      ],
      [
        14
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ],
      [
        15
      ]
    ]
  }
]
Img 20171030 190259
Created by

Rodrigo Gomez


Category

Plants

Compatible Instruments

MultispeQ beta


Latest Update

Aug 2017