multiwavelegnth LIRK with SPs and DIRKs pre-illuminated


test the effects of high light on IR, ECS and fluorescence with 2 dirks

pre-illumiknated for 60s

[
  {
    "v_arrays": [
      [
        20,
        30,
        30,
        10,
        10,
        10,
        10,
        10,
        10,
        10,
        10
      ],
      [
        10000,
        10000,
        10000,
        10000,
        10000
      ],
      [
        1,
        6,
        8,
        9,
        3
      ],
      [
        3,
        1,
        1,
        1,
        1
      ]
    ],
    "set_repeats": 10,
    "_protocol_set_": [
      {
        "do_once": 1,
        "pre_illumination": [
          2,
          "@s0",
          60000
        ],
        "autogain": [
          [
            0,
            1,
            3,
            30,
            50000
          ],
          [
            1,
            6,
            1,
            30,
            50000
          ],
          [
            2,
            8,
            1,
            30,
            50000
          ],
          [
            3,
            9,
            1,
            30,
            50000
          ],
          [
            4,
            10,
            1,
            70,
            20000
          ]
        ]
      },
      {
        "label": "ftrans",
        "pre_illumination": [
          2,
          "@s0",
          "@n1:0"
        ],
        "pulses": [
          10,
          10,
          100,
          10,
          100,
          10,
          10,
          10
        ],
        "pulses_delay": [
          [
            10
          ],
          [
            10
          ],
          [
            200
          ],
          [
            10
          ],
          [
            200
          ],
          [
            10
          ],
          [
            10
          ],
          [
            10
          ]
        ],
        "nonpulsed_lights": [
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ],
          [
            2
          ]
        ],
        "pulse_distance": [
          10000,
          10000,
          10000,
          10000,
          10000,
          10000,
          10000,
          10000
        ],
        "nonpulsed_lights_brightness": [
          [
            "@s0"
          ],
          [
            25000
          ],
          [
            2500
          ],
          [
            0
          ],
          [
            2500
          ],
          [
            0
          ],
          [
            25000
          ],
          [
            "@s0"
          ]
        ],
        "pulsed_lights": [
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ],
          [
            1,
            6,
            8,
            9,
            3
          ]
        ],
        "detectors": [
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ],
          [
            3,
            1,
            1,
            1,
            1
          ]
        ],
        "pulsed_lights_brightness": [
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ],
          [
            "a_b0",
            "a_b1",
            "a_b2",
            "a_b3",
            800
          ]
        ],
        "pulse_length": [
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ],
          [
            "a_d0",
            "a_d1",
            "a_d2",
            "a_d3",
            20
          ]
        ],
        "protocol_averages": 1,
        "protocol_repeats": 3
      }
    ]
  }
]
Dave in cap img 4615
Created by

David M. Kramer


Category

Algae
Connected Macro

simple multiwavelength LIRK


Compatible Instruments

MultispeQ v1.0 MultispeQ v2.0


Latest Update

Jan 2019