MultispeQ v1.0 Leaf Photosynthesis (Masked)


Works with the MultispeQ v1.0 ONLY

This is a shortened protocol to measure chlorophyll fluorescence and chlorophyll content when the light guides have been masked for small leaves.

This protocol will accurately measure plant physiological paramters (Phi2, PhiNPQ, PhiNO, qL, LEF, and relative chlorophyll content) and environmental parameters (light intensity, ambient temperature, ambient humidity and ambient pressure).

[
  {
    "pulses": [
      100,
      20,
      30,
      10,
      10,
      10,
      30,
      20,
      400,
      150,
      40,
      10,
      10,
      10,
      10,
      10,
      10
    ],
    "nonpulsed_lights": [
      [
        2
      ],
      [
        2
      ],
      [
        2
      ],
      [
        2
      ],
      [
        2
      ],
      [
        2
      ],
      [
        2
      ],
      [
        2
      ],
      [
        9
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ]
    ],
    "nonpulsed_lights_brightness": [
      [
        "light_intensity"
      ],
      [
        "light_intensity"
      ],
      [
        4500
      ],
      [
        4050
      ],
      [
        3600
      ],
      [
        3150
      ],
      [
        4500
      ],
      [
        "light_intensity"
      ],
      [
        2090
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ],
      [
        0
      ]
    ],
    "pulse_distance": [
      10000,
      10000,
      10000,
      10000,
      10000,
      10000,
      10000,
      10000,
      10000,
      10000,
      2000,
      2000,
      2000,
      2000,
      2000,
      2000,
      2000
    ],
    "pulsed_lights_brightness": [
      [
        0
      ],
      [
        2000
      ],
      [
        2000
      ],
      [
        2000
      ],
      [
        2000
      ],
      [
        2000
      ],
      [
        2000
      ],
      [
        2000
      ],
      [
        0
      ],
      [
        2000
      ],
      [
        0
      ],
      [
        450
      ],
      [
        700
      ],
      [
        3000
      ],
      [
        2250
      ],
      [
        7000
      ],
      [
        2250
      ]
    ],
    "pulse_length": [
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        30
      ],
      [
        20
      ],
      [
        20
      ],
      [
        20
      ],
      [
        20
      ],
      [
        20
      ],
      [
        20
      ],
      [
        20
      ]
    ],
    "detectors": [
      [
        0
      ],
      [
        1
      ],
      [
        1
      ],
      [
        1
      ],
      [
        1
      ],
      [
        1
      ],
      [
        1
      ],
      [
        1
      ],
      [
        0
      ],
      [
        1
      ],
      [
        0
      ],
      [
        3
      ],
      [
        1
      ],
      [
        3
      ],
      [
        1
      ],
      [
        3
      ],
      [
        1
      ]
    ],
    "pulsed_lights": [
      [
        0
      ],
      [
        3
      ],
      [
        3
      ],
      [
        3
      ],
      [
        3
      ],
      [
        3
      ],
      [
        3
      ],
      [
        3
      ],
      [
        0
      ],
      [
        3
      ],
      [
        0
      ],
      [
        2
      ],
      [
        6
      ],
      [
        2
      ],
      [
        6
      ],
      [
        2
      ],
      [
        6
      ]
    ],
    "recall": [
      "colorcal_blank1[1]",
      "colorcal_blank1[2]",
      "colorcal_blank1[3]",
      "colorcal_blank1[4]",
      "colorcal_blank1[6]",
      "colorcal_blank1[8]",
      "colorcal_blank1[9]",
      "colorcal_blank1[10]",
      "colorcal_blank2[1]",
      "colorcal_blank2[2]",
      "colorcal_blank2[3]",
      "colorcal_blank2[4]",
      "colorcal_blank2[6]",
      "colorcal_blank2[8]",
      "colorcal_blank2[9]",
      "colorcal_blank2[10]",
      "colorcal_blank3[1]",
      "colorcal_blank3[2]",
      "colorcal_blank3[3]",
      "colorcal_blank3[4]",
      "colorcal_blank3[6]",
      "colorcal_blank3[8]",
      "colorcal_blank3[9]",
      "colorcal_blank3[10]",
      "colorcal_intensity1_slope[2]",
      "colorcal_intensity1_yint[2]",
      "colorcal_intensity2_slope[2]",
      "colorcal_intensity2_yint[2]",
      "colorcal_intensity3_slope[2]",
      "colorcal_intensity3_yint[2]",
      "ir_baseline_slope[5]",
      "ir_baseline_yint[5]",
      "ir_baseline_slope[3]",
      "ir_baseline_yint[3]"
    ],
    "averages": 1,
    "open_close_start": 1,
    "environmental": [
      [
        "light_intensity"
      ],
      [
        "temperature_humidity_pressure"
      ],
      [
        "contactless_temp"
      ],
      [
        "temperature_humidity_pressure2"
      ],
      [
        "compass_and_angle"
      ]
    ]
  }
]
Default avatar
Created by

Sean


Category

Plants

Compatible Instruments

MultispeQ v1.0 MultispeQ v2.0


Latest Update

Sep 2017