measurements of dark adapted leaves for Phi2 under short time followed with a same time without illumination. After that a PMF measurement is done.
- Phi2 FIX ACTINIC 250 - RG - WITHOUT FR
This protocol allow you to follow changes on Phi2 over time with fixed actinic light set at 250 uE. The total time of the protocol is about 1 minute. Difference from the previous protocols is that this one exclude the final far-red oxidation allowing to observe the F0' increase after illumination. This will be useful to asses antimycin A effect.
- Phi2 FIX ACTINIC 0 - RG - WITHOUT FR
This protocol allow you to follow changes on Phi2 over time under darkconditions. The total time of the protocol is about 50 S
- Proton Motive Force via DIRK fixed actinic II
10 averages instead of 5, lower light intensity on actinic. higher intensity on measuring pulse