Disease, Damage, and Drought: Impacts on Photosynthesis

Overview

Questions

  • How does photosynthesis and other measurements change in diseased, damaged, and otherwise unhappy plants?

  • What diseases impact photosynthesis, and how do they do it?

  • Is it possible to identify the onset of diseases (especially non-visible diseases like root rots) using photosynthesis measurements?

Goal

The goal of this project is to survey of ‘unhappy’ plants and comparison to similar ‘happy’ plants to identify the ways in which photosynthesis changes based on different types of disease, drought, and damage. By collecting information from many environments across the world, we may be able to develop early predictors of these stresses and better understand how plants adjust and defend themselves.

Directions

NOTE: Make sure NOT to measure holes or tears directly - if there are physical holes measure right next to the hole, not on top of it. Ideally, each measurement should be on a slightly different part of the leaf, or different leaves all together.

  1. Measure a diseased plant or leaf 5 times.
  2. Measure a healthy leaf 5 times on the same plant, or a plant of the same species nearby.

You'll also need to know roughly what has damaged the leaf. Here are some examples:

Pathogens:

Viruses, bacterial, and fungal- including root rots not visible on foliage

Pathogens.png

Pests:

Insects and pest animals

Pests.png

Weather:

Frost, drought, heat, wind, and flooding

weather.png

Physical:

Gonstruction, human, and other physical

physical.png

Protocols

Leaf Photosynthesis MultispeQ V1.0

Summary

Measures many photosynthesis-related parameters in <15 seconds, including:

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF
  • Relative Chlorophyll: SPAD
  • Proton Motive Force: ECSt, vH+, gH+
  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.
  • Leaf Thickness (in mm), angle, and cardinal direct...

Questions

  1. Plant Type (Multiple Choice with images)
  2. Plant Height (Multiple Choice)
  3. Optional: Genus and Species (lowercase) (Short Answer)
  4. Leaf Condition (Multiple Choice with images)
  5. Optional: Disease or Pest Name (Short Answer)
  6. Leaf Location (Multiple Choice)
  7. Leaf Image (Take a Picture)
  8. Leaf Number (Use Auto Increment) (Multiple Choice)

Locations

Team

Thumb sam 0645   copy   copy  2
Project Lead (0)
Thumb p6030740
Collaborator (30)
Thumb 012409 pbi 160 low low res headshot
Collaborator (65)
Default avatar
Collaborator (30)
Thumb img 20160320 094226
Collaborator (1)
Default avatar
Collaborator (30)
Default avatar
Collaborator (0)
Default avatar
Collaborator (0)
Default avatar
Collaborator (0)
Thumb hf3a9870
Collaborator (0)
Default avatar
Collaborator (0)
Default avatar
Collaborator (0)
Default avatar
Collaborator (0)
Thumb screen shot 2017 05 31 at 12.23.10 pm
Collaborator (0)