Fluorescence Fv/Fm and PSII Yield


Analyze: Chlorophyll fluorescence; Fv/Fm and PSII Yield with ambient light; Macro
//============================================
// Macro for data evaluation on PhotosynQ.org
// created: 27.10.2017
//============================================

var data = json.data_raw;
var output = {};
var Fs = {};
var PhiII = {};
var baseline = 0;

var light_intensity =  {};

// retrieve the baseline information from the data JSON and save (using measuring light 15, calibrating light 14)

var sample_cal = json.detector_read1;//es para la luz 940

var shinyness = (sample_cal-json.recall["ir_baseline_yint[5]"])/json.recall["ir_baseline_slope[5]"]; // where 0 is dull black electrical tape, and 1 is shiny aluminum
var baseline = json.recall["ir_baseline_slope[3]"]*shinyness+json.recall["ir_baseline_yint[3]"];

if (!baseline || !json.recall["ir_baseline_yint[5]"] || sample_cal == 65535) {						// if it hasn't been calibrated or there's an error or it's maxed, set baseline == 0
  baseline = 0;
}



// initial values of F0 and Fm from dark adapted state.

var F0 = MathMEAN(data.slice(20,69)) - baseline;
var Fm = MathMEAN(data.slice(100,135)) - baseline;
var fvfm = (Fm-F0)/Fm;


var F0P = MathMEAN(data.slice(4000,4150)) - baseline;
var FmP = MathMEAN(data.slice(4180,4250)) - baseline;
var Yield = (FmP-F0P)/FmP;


output ["F0"] = MathROUND(F0,0);
output ["Fm"] = MathROUND(Fm,0);
output ["Fv/Fm"] = MathROUND(fvfm,3);

output ["F0P"] = MathROUND(F0P,0);
output ["FmP"] = MathROUND(FmP,0);
output ["Yield"] = MathROUND(Yield,3);




     
    

    
 //output ["Fs " + i] = MathROUND(Fs [i], 0);
 //output ["Fm' " + i] = MathROUND(FmP [i],0);
 //output ["F0' calc " + i] = MathROUND(f0p [i],0);
 //output ["PhiII " + i] = MathROUND(PhiII [i],3);
 

          




var thickness = json.thickness;
output ["thickness - mm "] = MathROUND(thickness , 4);

output ["baseline" ] = baseline;

output["Light Intensity (PAR)"] = json.light_intensity;
output["Temperature"] = json.temperature;
output["Humidity"] = json.humidity;
output["Pressure"] = json.pressure;
return output;
{
  "time_offset": -120,
  "time": 1509107557999,
  "device_name": "MultispeQ",
  "device_version": "1",
  "device_id": "01:06:11:94",
  "device_battery": 80,
  "device_firmware": 1.17,
  "sample": [
    {
      "time": 1509107558032,
      "protocol_id": 1,
      "detector_read1": 13798,
      "light_intensity": 23,
      "r": 19,
      "g": 8,
      "b": 7,
      "light_intensity_raw": 30,
      "temperature": 25.1,
      "humidity": 37.606445,
      "pressure": 1009.148254,
      "temperature2": 25.459999,
      "humidity2": 39.138672,
      "pressure2": 1010.255371,
      "contactless_temp": 24.85,
      "thickness": 0.24,
      "compass_direction": "W",
      "compass": "270.00",
      "angle": 2.52,
      "angle_direction": "N",
      "pitch": 2.43,
      "roll": -0.64,
      "recall": {
        "time": 1509107601929,
        "colorcal_blank1[1]": 22499.5,
        "colorcal_blank1[2]": 25554,
        "colorcal_blank1[3]": 31458,
        "colorcal_blank1[4]": 38876.5,
        "colorcal_blank1[6]": 34085.5,
        "colorcal_blank1[8]": 33723,
        "colorcal_blank1[9]": 30423.5,
        "colorcal_blank1[10]": 36190,
        "colorcal_blank2[1]": 21669.5,
        "colorcal_blank2[2]": 18635,
        "colorcal_blank2[3]": 22455,
        "colorcal_blank2[4]": 19658.5,
        "colorcal_blank2[6]": 16528,
        "colorcal_blank2[8]": 17891.5,
        "colorcal_blank2[9]": 18626,
        "colorcal_blank2[10]": 16696,
        "colorcal_blank3[1]": 20402,
        "colorcal_blank3[2]": 10194,
        "colorcal_blank3[3]": 19539.5,
        "colorcal_blank3[4]": 14416,
        "colorcal_blank3[6]": 5821.5,
        "colorcal_blank3[8]": 6505,
        "colorcal_blank3[9]": 5453.5,
        "colorcal_blank3[10]": 5999,
        "colorcal_intensity1_slope[2]": 2.626,
        "colorcal_intensity1_yint[2]": 0.7,
        "colorcal_intensity2_slope[2]": 2.605,
        "colorcal_intensity2_yint[2]": -2.1,
        "colorcal_intensity3_slope[2]": 2.666,
        "colorcal_intensity3_yint[2]": -19.700001,
        "ir_baseline_slope[5]": 13602,
        "ir_baseline_yint[5]": 20124,
        "ir_baseline_slope[3]": 564,
        "ir_baseline_yint[3]": 751
      },
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  ],
  "app_os": "win",
  "app_name": "PhotosynQ",
  "app_version": "0.3.9",
  "app_device": "x86-32",
  "location": [
    "50.1563532",
    "8.6352638"
  ],
  "ConsoleMacro": null
}
Default avatar
Created by

Lars Dietzel


Protocol connections:
1
Latest Update:
Oct 2017