Active carbon in soil, Calibration, Modified 15mM KMnO4 version V1.0


Used with "Active carbon in soil calibration", see protocol for more detailed information. Saves calibration slope and yintercept values to location userdef7 (1029+).
// a linear regression is made from the raw values subtracted from the blank.  In addition, the log relationship is also calculated and outputted.

var output = {};

// save the user identied concentrations for solutions 1 - 3

/// divide by 1000 to convert to mM from M


var solution1 = Number(json.message[0][2])/1;
var solution2 = Number(json.message[4][2])/1;
var solution3 = Number(json.message[8][2])/1;

var I0 = json.recall[userdef[6]]; // get blank

var Step11 = MathROUND(MathMEAN(json.data_raw.slice(40,460)),2);
var Step12 = MathROUND(MathMEAN(json.data_raw.slice(540,960)),2);
var Step13 = MathROUND(MathMEAN(json.data_raw.slice(1040,1460)),2);

var Step21 = MathROUND(MathMEAN(json.data_raw.slice(1540,1960)),2);
var Step22 = MathROUND(MathMEAN(json.data_raw.slice(2040,2460)),2);
var Step23 = MathROUND(MathMEAN(json.data_raw.slice(2540,2960)),2);

var Step31 = MathROUND(MathMEAN(json.data_raw.slice(3040,3460)),2);
var Step32 = MathROUND(MathMEAN(json.data_raw.slice(3540,3960)),2);
var Step33 = MathROUND(MathMEAN(json.data_raw.slice(4040,4460)),2);

var I1 = (Step11+Step12+Step13)/3;
var I2 = (Step21+Step22+Step23)/3;
var I3 = (Step31+Step32+Step33)/3;



// do it using absorbance

var A1 = MathLOG( ( I1/I0 ) ) * -1;
var A2 = MathLOG( ( I2/I0 ) ) * -1;
var A3 = MathLOG( ( I3/I0 ) ) * -1;
var reg = MathLINREG([A1,A2,A3],[solution1,solution2,solution3]);   // measured in micromolar

output["toDevice"] = "1029+"+MathROUND(reg.m,6)+"+"+MathROUND(reg.b,6)+"+-1+";
output["slope"] = MathROUND(reg.m,6);
output["y-int"] = MathROUND(reg.b,6);
output["r2"] = MathROUND(reg.r2,6);
output["A1"] = A1;
output["A2"] = A2;
output["A3"] = A3;
output["solution1"] = solution1;
output["solution2"] = solution2;
output["solution3"] = solution3;
output["message"] = json.message;



var r_A1 = MathROUND(I0 - I1,4);
var r_A2 = MathROUND(I0 - I2,4);
var r_A3 = MathROUND(I0 - I3,4);
var r_reg = MathLINREG([solution1,solution2,solution3],[r_A1,r_A2,r_A3]);   // measured in micromolar

//output["toDevice"] = "1029+"+MathROUND(r_reg.m,6)+"+"+MathROUND(r_reg.b,6)+"+-1+";
output["r_slope"] = MathROUND(r_reg.m,6);
output["r_y-int"] = MathROUND(r_reg.b,6);
output["r_r2"] = MathROUND(r_reg.r2,6);
output["r_A1"] = r_A1;
output["r_A2"] = r_A2;
output["r_A3"] = r_A3;


// do it for transmittence

var t_A1 = MathROUND(I1/I0,4);
var t_A2 = MathROUND(I2/I0,4);
var t_A3 = MathROUND(I3/I0,4);

var t_reg = MathLINREG([t_A1,t_A2,t_A3],[solution1,solution2,solution3]);   // measured in micromolar

output["toDevice"] = "1029+"+MathROUND(t_reg.m,6)+"+"+MathROUND(t_reg.b,6)+"+-1+";
output["t_slope"] = MathROUND(t_reg.m,6);
output["t_y-int"] = MathROUND(t_reg.b,6);
output["t_r2"] = MathROUND(t_reg.r2,6);
output["t_A1"] = t_A1;
output["t_A2"] = t_A2;
output["t_A3"] = t_A3;
*/
//var sample = (As - reg.b)/reg.m

//var activeCarbon = (0.02 - (reg.b +(reg.m * As))) * 9000 * (0.02 / 0.00248)

//Active Carbon (mg kg -1) = [0.02 mol/ L – (a + b × Abs)] × (9000 mg C/ mol) × (0.02 L solution/ Wt)
//[0.02 M – (-0.00004 + (0.0502 × 0.3087)] × (9000 mg C/ mol) × (0.02 L solution/ 0.00248 kg) = 329.75 mg POXC kg -1 soil

//output["f(x)log"] = ["y = " + MathROUND(reg.m,6)+" x +" + MathROUND(reg.b,6)," r2: " + MathROUND(_reg.r2,2)];

//output["f(x)linear"] = ["y = " + MathROUND(_reg.m,6)+" x +" + MathROUND(_reg.b,6)," r2: " + MathROUND(_reg.r2,2)];

output["blank"] = I0;
output["I1"] = I1;
output["I2"] = I2;
output["I3"] = I3;

return output;
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  "time_offset": 300,
  "time": 1483645967618,
  "device_version": "1",
  "device_id": "d4:f5:00:00:00:11",
  "device_battery": 96,
  "device_firmware": "1.13",
  "firmware_version": "1.13",
  "device_time": 1479297288,
  "sample": [
    [
      {
        "time": 1483645967631,
        "protocol_id": 1,
        "message": [
          [
            "0",
            "0",
            ""
          ],
          [
            "prompt",
            "Enter 100 pct standard  solution concentration in mM note if following standard calibration enter 15",
            ""
          ],
          [
            "confirm",
            "Insert solution 1 1 of 3",
            "ok"
          ],
          [
            "confirm",
            "Reinsert soln 1 2 of 3",
            "ok"
          ],
          [
            "confirm",
            "Reinsert soln 1 3 of 3",
            "ok"
          ],
          [
            "prompt",
            "Enter 50 pct standard  solution concentration in mM note if following standard calibration enter 7point5",
            ""
          ],
          [
            "confirm",
            "Insert solution 2 1 of 3",
            "ok"
          ],
          [
            "confirm",
            "Reinsert soln 2 2 of 3",
            "ok"
          ],
          [
            "confirm",
            "Reinsert soln 2 3 of 3",
            "ok"
          ],
          [
            "prompt",
            "Enter 25 pct standard  solution concentration in mM note if following standard calibration enter 3point75",
            ""
          ],
          [
            "confirm",
            "Insert solution 3 1 of 3",
            "ok"
          ],
          [
            "confirm",
            "Reinsert soln 3 2 of 3",
            "ok"
          ]
        ],
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      }
    ]
  ],
  "app_os": "win",
  "app_name": "PhotosynQ",
  "app_version": "0.3.5",
  "app_device": "x86-32",
  "location": [
    "42.7225667",
    "-84.4746410"
  ],
  "ConsoleMacro": "145"
}
Default avatar
Created by

Sean


Protocol connections:
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Latest Update:
Jan 2017