Screen orange fleshed sweetpotato clones for drought

Overview

Due to climate change, drought problems are on the increase in Malawi. For this reason, breeders are developing new sweet potato varieties with an emphasis on drought tolerance. This project aims at research-farmer participatory screening and selecting of orange fleshed sweet potato genotypes for drought tolerance. The selected lines/genotypes will be used as:

(1) Parental material for hybridization

(2) Drought tolerant genotypes for production in Malawi

The trial has two water levels; water-stressed and non water-stressed. In the stressed, irrigation is stopped at six weeks after planting while in non stressed, irrigation continues up to near physiological maturity.

This project is testing 22 lines, which includes lines introduced from Mozambique. Data will be collected at 3 weeks intervals from the sixth week after planting up to physiological maturiry.

Protocols

The One Protocol (Phi2, PSI, NPQ) II

The One Protocol to rule them all! Phi2, PSI, NPQt, combined!

Changes from previous The One protocol: - no longer outputs signal from the far red period, which simplifies the signal output for the user - changed distance between measuring pulses from 10,000 to 2,000 which improves measurement resolution - has an initial stabilization period, so Fs has a chance to adjust to the actinic. - the measuring lights are used only when actually needed (before lights 15 and 10 were always pulsed, even when the values were not used in the macro outputs) - removed light 2 (650) on add-on board during the actinic periods so now actinic is just light 20 (650) on the main boards. This was causing the actinic to not match ambient, as the combination of two 650 lights was greater than the ambient value.

Chlorophyll content (SPAD) III

REQUIREMENTS: Firmware v0.41 or higher. Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness. Improvements over Chlorophyll content (SPAD): - performs 3 different spads at 3 intensities for thin and thick leaves - auto-chooses appropriate intensity - is calibrated to a Minolta SPAD device from factory.

Improvements over previous version -

  • Compatible with firmware v0.41 or higher.

Questions

  1. Stress
  2. Rep
  3. Weeks after planting
  4. Variety
  5. leaf position
  6. Year
  7. site

Locations

Discussions


Team

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