NPQ PMF tabaco RG II

Overview

NPQ measurements of tobacco plants

Protocols

NPQ Short RG CORR2

Using an orange measuring light (pin 15) and red saturating light (pin 20), this performs the standard PAM fluorescence measurement to obtain the decay kinetics and NPQ values from a short period of time.
CORRECTED LAST PULSE

Proton Motive Force via DIRK fixed actinic II

10 averages instead of 5, lower light intensity on actinic. higher intensity on measuring pulse

Chlorophyll content (SPAD) III

REQUIREMENTS: Firmware v0.41 or higher. Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness. Improvements over Chlorophyll content (SPAD): - performs 3 different spads at 3 intensities for thin and thick leaves - auto-chooses appropriate intensity - is calibrated to a Minolta SPAD device from factory.

Improvements over previous version -

  • Compatible with firmware v0.41 or higher.

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