Common bean drought in DEPI chambers

Overview

We are measuring photosynthetic parameters of three common bean verities of differing drought tolerance using MSU's CAAPP center DEPI imagers. Trying photosynQ to determine potential for screening in field and previously uncollected measurements for screening potential.

Protocols

Chlorophyll content (SPAD) I

REQUIREMENTS: Firmware v0.38 or lower (used for older devices which do not have a SPAD calibration, generally device ID 87 and lower).Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness.

The One Protocol (Phi2, PSI, NPQ) II

The One Protocol to rule them all! Phi2, PSI, NPQt, combined!

Changes from previous The One protocol: - no longer outputs signal from the far red period, which simplifies the signal output for the user - changed distance between measuring pulses from 10,000 to 2,000 which improves measurement resolution - has an initial stabilization peri...

Proton Motive Force via DIRK

Estimates the proton motive force through the ATP synthase, thereby estimating ATP production. The proton motive force is measured by measuring the electrochromic shift (ECS), the shift in the absorbance of chlorophyll at ~520nm in the thylakoid membrane due to a shift in the electric field. The electric field is created by the accumulation of...

Questions

  1. Time of measurement (Multiple Choice)
  2. Bean Variety (Multiple Choice)
  3. Leaf type (Multiple Choice)
  4. Leaf number (Multiple Choice)
  5. Plant watering idenity (Multiple Choice)

Team

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