We are using this page for students to practice using the multispeQ device and to collect data from trees. Students get experience making observations so they can come up with different a variety of questions they might consider asking in their research projects.
We are going to collect data from Quercus imbricaria trees based on this criteria: - tree code - cardinal direction (northeast, southeast, southwest or northwest) - branch zone (lower, middle or top) - random leaf sample (1 to 10) - random measurement # per leaf (1 to 10) - leaf color (dark green, green, pale green, green-yellow, green-red, yellow-green, yellow-red, red, red-green, red-yellow, orange, orange-red, orange yellow, rust red, brown, brown-yellow, brown green etc.)
Measurement Instructions If using Android pad or Nexus, turn the wifi off before going outside.
Open the measuring part of the device so it can acclimate to the temperature. When you get to the tree, turn on the device and be sure to pair or synch it to the pad.
Random Sampling Method: Randomly sample 10 dry outside leaves in each quadrant based on cardinal direction of three different trees of the same species during fall.
Find tree and select the right tree code on the app.
Orient yourself so you can see the tree divided into 4 quadrants (e.g., northeast, southeast, southwest and northwest).
Start sampling in the northeast quadrant and set the app question to this.
Close your eyes and randomly sample one dry, outside leaf in this quadrant.
Select the color of the leaf. For multicolored leaves, 1st color is for the more sizable color and second is the less sizable color. If there are 3 or more colors then select that choice.
Attach the device to the TOP of a DRY leaf so the leaf is held in its natural "bend". Make sure that you are not blocking the white square (light detector). Once you have attached the device to the leaf, immediately take the measurement.
Wait until you see a graph of the data collected before removing the device from the leaf.
Confirm that the data is in the expected ranges (phi2 0.2 to 0.85 and SPAD of 0 to 85) and also confirm that the graph has the expected curve (drop and rise). All looks, good then keep it; otherwise discard. Click the okay to save data to the pad.
Repeat step 4 on nine more leaves in that quadrant (remember to always change the leaf color when necessary). Goal is to sample 10 outside leaves per quadrant per tree.
Move to the southeast quadrant, change the quadrant section and repeat steps 4 and 5. Continue doing the same process for the remaining two quadrants in that tree, making sure you first change the quadrant and always change the leaf color when necessary.
Move to the next tree and repeat steps 4 to 6.
To upload the data, reconnect the wifi and then go back to the list of projects and click on the refresh arrow. You should get a note about the data being uploaded.
Protocols: The One v3.0 (Phi2, NPQt, using Multi-Phase Flash) The One Protocol to rule them all! Phi2, NPQt combined. Changes from previous The One protocol: - Removed the Phi1 measurement, which was producing a lot of invalid data (removed by Dan and Frank) - Implemented the multi-phase flash protocol created by Tom Avenson
Chlorophyll content (SPAD) III REQUIREMENTS: Firmware v0.41 or higher. Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness. Improvements over Chlorophyll content (SPAD): - performs 3 different spads at 3 intensities for thin and thick leaves - auto-chooses appropriate intensity - is calibrated to a Minolta SPAD device from factory. Improvements over previous version - - Compatible with firmware v0.41 or higher.
- Leaf Photosynthesis - MultispeQ Beta ONLY
Measures photosynthesis-related parameters in <15 seconds, including: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF, and SPAD. In addition, measures PAR (photosynthetically active radiation), ambient temperature and relative humidity.
Works with the MultispeQ Beta device only
- Quercus imbricaria tree code was? (Multiple Choice)
- Which quadrant sampled? (Multiple Choice)
- Tree branch location was in which portion of whole tree? (Multiple Choice)
- Leaf Color was? (Multiple Choice)
- Randomly selected leaf counted in the quadrant -- # count? (Multiple Choice)