Bean germplasm screening for tolerance to moisture stress

Overview

We screened 85 different lines of common bean for moisture stress under full and deficit irrigation at the Lilongwe University of Agriculture and Natural Resources

Protocols

The One v3.0 (Phi2, NPQt, using Multi-Phase Flash)

The One Protocol to rule them all! Phi2, NPQt combined.

Changes from previous The One protocol: - Removed the Phi1 measurement, which was producing a lot of invalid data (removed by Dan and Frank) - Implemented the multi-phase flash protocol created by Tom Avenson

Chlorophyll content (SPAD) III

REQUIREMENTS: Firmware v0.41 or higher. Measures transmission at 940nm and 650nm in order to determine the 'greenness' of a leaf, an indicator of chlorophyll content and nitrogen content. The 940 is used to account for leaf thickness. Improvements over Chlorophyll content (SPAD): - performs 3 different spads at 3 intensities for thin and thick leaves - auto-chooses appropriate intensity - is calibrated to a Minolta SPAD device from factory.

Improvements over previous version -

  • Compatible with firmware v0.41 or higher.

Questions

  1. moisture management (Multiple Choice)
  2. Rep (Multiple Choice)
  3. weeks after last watering in stressed (Short Answer)
  4. Tier (Multiple Choice)
  5. Row (Multiple Choice)
  6. leaf positon (Multiple Choice)

Locations

Discussions

Team

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