5 - v1.0 FACTORY CALIBRATION

Overview

Description

Do not recalibrate you device unless you know what you're doing! If you have questions or concerns about your device, please ask on the forums

This calibration includes:

  1. Final confirmation using Leaf Photosynthesis protocol on 3 plants and 3 leaves under different conditions.

Directions

  1. Measure 3 leaves on 3 plants, noting the plant and leaf #.
  2. Confirm that the results are in the expected range.

Protocols

Leaf Photosynthesis MultispeQ V1.0

Summary

Measures many photosynthesis-related parameters in <15 seconds, including:

  • Chlorophll Fluorescence: Phi2, PhiNPQ, PhiNO, NPQt, qL, LEF
  • Relative Chlorophyll: SPAD
  • Proton Motive Force: ECSt, vH+, gH+
  • Absorbance at: 450, 535, 605, 650, 730, 850, 880, and 940nm.
  • Leaf Thickness (in mm), angle, and cardinal direction
  • Leaf Temperature and differential from ambient temperature
  • Environmental conditions: PAR and ambient temperature/pressure/humidity

Description

Leaf Photosynthesis MultispeQ V1.0 is the classic and by far the most utilized PhotosynQ Protocol.

This protocol delivers a wide range of plant photosynthetic parameters, a full list can be found here

If you want to see the exact calculations for each parameter, please see the associated macro called "Leaf Photosynthesis MultispeQ V1.0".

There are two publications about the MultispeQ Beta device covering many of parameters and their comparisons to commercial instruments. While the beta was an older model device, the calculations and comparisons are still true for this protocol and V1.0 device. You can find them at the Royal Society for Open Science, and Plant, Cell & Environmental Journal.

The Leaf Photosynthesis protocol collects many different measurements from a leaf as quickly as possib...

Questions

  1. Leaf Number (Multiple Choice)

Locations

Discussions

Team

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