Non-Photochemical Quenching

Education

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Hi folk! NPQ it's (qE + qT + qI); source (http://www.plantphysiol.org/content/125/4/1558.short)

Can we use MultispeQ for calculate qE for example?

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Hi, I started building a protocol but abandoned it for now to work on other things. The protocol works, you can see some output data in project but the macro does not do all calculations yet.

project https://photosynq.org/projects/npq-recovery-b-rapa-drought-e3521393-b81e-4f9e-af76-4fcc7cf4feac

Feel free to take a look at project and protocol.

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David M. Kramer

Jan 2018

Hi all, The new RIDES protocols, possible with Firmware 2.2 and up, make this much easier to do. Stay tuned! Dave

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Wojciech Baba

28 days ago

I have a Multispeq v1.0 with firmware 1.17 - where to find and how to install firmware 2.2 ?

I think David meant 1.22

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Wojciech Baba

28 days ago

Thank You. However, on https://github.com/Photosynq/PhotosynQ-Firmware/releases I the latest beta version is 1.21 (without .hex file). Would be so kind to give me any advice how to install it on new Multispeq 1.0 ?

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Sebastian

27 days ago

We just added a new chapter in the help center on how to install firmware updates. So please check out How to Install Firmware Updates.

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Wojciech Baba

27 days ago

Thank You very much. And it is possible to use RIDES protocol on existing firmware (i.e. version 1.21) ?

Thank you, David. I will try!

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Sebastian

Dec 2017

Yes, you absolutely can!

You have to create a protocol, to do the dark adaptation, to measure the fluorescence kinetics under constant light and the dark recovery. It is a little bit tricky, but we have done these measurements in the past with the MultispeQ beta.

Please let me know if you need some help building the protocol.